SEARCH:   Advanced

Blood, Vol. 109, Issue 5, 1850-1856, March 1, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Postgenomic up-regulation of CCL3L1 expression in HTLV-2–infected persons curtails HIV-1 replication Blood Pilotti et al. 109: 1850 Supplemental materials for: Pilotti et al, Vol 109, Issue 5, 1850-1856 Files in this Data Supplement: Document S1. Supplemental materials and methods (PDF, 22.1 KB) Document S2. Supplemental data (PDF, 65.7 KB) Table S1. Cell-surface expression of CCR5 on PBMC subsets cultivated in the presence or absence of anti-CCR5 binding chemokine NmAb (PDF, 21.7 KB) - Relative changes in mean fluorescence intensity (MFI) cell-surface expression of CCR5 at day 6 of culture were calculated as (MFIsti-spe – MFIsti-con)/(MFImed-spe – MFImed-con) × 100. MFIsti-spe and MFIsti-con treated cells labeled with specific anti-CCR5 of NmAbs and with isotype-matched control IgG, respectively. MFImed-spe and MFImed-con are MFI of NmAbs untreated cells. The results show the range of 5 independent experiments involving cells from 4 HTLV-2/HIV-1MEU persons. Table S2. Effect of HIVgp120 Env-C5-peptide on CC chemokine production by PBMCs of HTLV-2/HIV-1MEU persons (PDF, 22.7 KB) - The results are the mean of 5 independent experiments done with PBMCs from 4 HTLV-2/HIV-1MEU persons. The C5-peptide (10 µg/mL) was added once at the time of cell seeding. *SPI (spontaneous proliferation index) was determined by 3H thymidine incorporation at day 2 after 24 hours of incubation. SPI level of the untreated cells is defined as 1 and used as the term of comparison. #CC chemokine production is expressed as pg/mL/106 cells. Table S3. Modulation of CCR5 expression in unstimulated PBMC cultures from HTLV-2/HIV-1MEU persons (PDF, 14.5 KB) - Immunophenotyping and CCR5 cell-surface expression were detected by flow cytometry. Results are representative of 3 independent experiments and are expressed as percentage of positive cells. Figure S1. R5 HIV-1 replication in unstimulated PBMCs from HTLV-2/HIV-1MEU subjects: dominant role of MIP-1α (PDF, 31.9 KB) - (A) Kinetics of R5 (ADA and IIIB) and X4 (BaL) virus production in IL-2–stimulated PBMCs obtained from a blood pool of uninfected donors. Supernatants from PBMC cultures were harvested every 4 to 6 days and tested for HIV-1 p24 gag Ag concentrations; the results are representative of 5 independent experiments performed at 6-month intervals. (B) Kinetics of R5 and X4 virus production by primary cultures obtained from HTLV-2/HIV-1MEU persons. Neutralizing activity of antichemokine mAbs was determined in the presence of each NmAbs (2 µg/mL) added in combination. The kinetics of virus production was measured in PBMCs infected at day 0 (C) or day 6 (D), in the presence and absence of anti–MIP-1 NmAb (2 µg/mL). Figure S2. Representative experiment of CC chemokine secretion by unstimulated PBMCs or PBMC subsets isolated from HTLV-2/HIV-1MEU persons (PDF, 24.8 KB) - (A) Kinetics of CC chemokine secretion in PBMC culture supernatants was determined by ELISA. (B) MIP-1α transcript level was analyzed by RT-PCR and normalized to that of the ACTB gene. (C) PBMCs from HTLV-2/HIV-1MEU subjects were separated into T lymphocytes and monocytes and for culture. Cell subset supernatants were harvested and quantified for CC chemokine production by ELISA. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020