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Blood, Vol. 109, Issue 8, 3225-3234, April 15, 2007
Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach
Blood Raslova et al.
109: 3225
Supplemental materials for: Raslova et al, Vol 109, Issue 8, 3225-3234
Files in this Data Supplement:
- Table S1. List of genes regulating by ploidy of MKs (XLS, 0.97 MB)
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ANOVA was performed on the 41 059 probes of the chips with P thresholds less than or equal to 10–5. Then 416 probes were obtained by comparing the 4N, 8N, and 16N MK populations with the 2N MK population; 943 probes were obtained by comparing the 2N, 4N, 8N, and 16N MK populations to a MK pool; and 367 probes were common between these two analyses.
- Table S2. List of genes discriminating between 2N, 4N and 8N, 16N MKs (XLS, 70 KB)
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Combined microarray data were exported from Resolver into BRB ArrayTools. Analysis of discriminating genes between the 2N-4N and 8N-16N groups was performed using the class prediction module with the random variance model. As data were previously normalized by feature extraction software, no spot filtering and no normalization were performed. Genes with more than 50% missing values were excluded. Finally, 105 genes were selected based on a P threshold (P ≤ .001).
- Table S3. Correlation between microarray and QRT-PCR methods (XLS, 30.5 KB)
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Pearson correlation between data corresponding to the expression levels obtained by QRT-PCR and by microarray analysis for 88 probes representing 61 genes.
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