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Blood, Vol. 109, Issue 3, 1275-1283, February 1, 2007
Impaired ribosome biogenesis in Diamond-Blackfan anemia
Blood Choesmel et al.
109: 1275
Supplemental materials for: Choesmel et al, Vol 109, Issue 3, 1275-1283
Files in this Data Supplement:
- Figure S1. Characterization of the fibroblasts from DBA patients (PDF, 107 KB) -
(A) Growth of fibroblasts from three DBA patients and two controls. All measures were performed in triplicate. The generation times were calculated by fitting the growth curves with an exponential equation (r2>0.99 in all cases). (B) Levels of RPS19 mRNA in control and DBA fibroblasts. cDNAs amplified from fibroblasts were submitted to quantitative PCR (ABI prism GeneAmp 5700 Sequence Detection System; Applied Biosystems) using SYBRgreen detection. Oligonucleotides were designed to reveal either the mRNAs synthesized from both wild type and mutant RPS19 alleles (dark grey bars), or only the mutated forms (light grey bars). No signal was detected in control cells with the mutated allele specific probes. The GADPH mRNA was used for normalization (primers available upon request).
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