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Blood, Vol. 109, Issue 11, 4899-4906, June 1, 2007
Development of a murine model for blastoid variant mantle-cell lymphoma
Blood Ford et al.
109: 4899
Supplemental materials for: Ford et al, Vol 109, Issue 11, 4899-4906
Files in this Data Supplement:
- Figure S1. Flow cytometry of tumors from IL-14α TG, DTG, and c-myc TG tumors showing forward and side light scatter, CD19, CD21, CD23, and sIgD, as described in “Flow cytometry” (JPG, 48.4 KB)
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- Figure S2. Cytogenetic study of a DTG lymphoma cell line (JPG, 20.7 KB)
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The 3:10 translocation and trisomy 15 are demonstrated. In addition, there is trisomy 16, trisomy 18, and a translocation involving chromosome 11 and loss of 1 each of chromosomes 8, 9, and 13. (Cytogenetics method: exponentially growing murine lymphoma cells were harvested for chromosome preparation following the standard Colcemid, hypotonic saline, and fixation laboratory procedures routinely used in our laboratory. Optimally aged slides were used for performing Giemsa G- banding and marker chromosome analysis, following the International System for Cytogenetic Nomenclature (ISCN) for mouse chromosomes. A minimum of 40 metaphases from each sample were counted for the model chromosome number, and at least 5 complete karyotypes were prepared for the identification of any structural or numerical chromosomal abnormality.)
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