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Blood, Vol. 109, Issue 11, 4924-4929, June 1, 2007
The myeloproliferative disorder–associated JAK2 V617F mutant escapes negative regulation by suppressor of cytokine signaling 3
Blood Hookham et al.
109: 4924
Supplemental materials for: Hookham et al, Vol 109, Issue 11, 4924-4929
Files in this Data Supplement:
- Figure S1. SOCS3 and pJAK2 are rescued by lysosome inhibitors (PDF, 46.6 KB) -
293T cells were transfected with erythropoietin receptor (EPOR) in combination with JAK2, FLAG-SOCS3, or both JAK2 and FLAG-SOCS3. At 48 hours after transfection, cells were treated with or without 25 µM E64D for 2 hours prior to lysis. Cell lysates were analyzed for pY JAK2 (top panel), JAK2 (second panel), SOCS3 (third panel), or  -tubulin (bottom panel).
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