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Blood, Vol. 110, Issue 1, 278-286, July 1, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Notch signals positively regulate activity of the mTOR pathway in T-cell acute lymphoblastic leukemia Blood Chan et al. 110: 278 Supplemental materials for: Chan et al, Vol 110, Issue 1, 278-286 Files in this Data Supplement: Table S1. Characteristics of T-cell leukemia lines used in this study (XLS, 22.5 KB) Table S2. Complete list of the antibodies used in this study (XLS, 87.5 KB) Table S3. Raw ratiometric data (XLS, 49.5 KB) - Ratios are logged in base 2 and median-centered for each cell line. Figure S1. Notch inhibition does not cause substantial changes in cell death (JPG, 67.9 KB) - Cell lines were treated with compound E (GSI) or vehicle (DMSO) for 7 days. Cell death in each population was measured by determining the fraction of cells with DNA content in the less-than-G1/G0 fraction after propidium iodide staining. Figure S2. Expression of dominant negative Mastermind-like-1 protein (DN-MAML-1) suppresses S6 ribosomal protein phosphorylation (JPG, 80.1 KB) - Indicated cell lines were transduced with MSCV-GFP-DN-MAML-1 or MSCV-GFP retroviruses. Eight days following transduction, cells were intracellularly stained for phospho-S6 RP and total S6 RP. (A) Level of phospho-S6 RP staining in GFP+ gated cells. Red histogram represents staining from MSCV-GFP transduced control cells and green histogram from DN-MAML-1 expressing cells. (B) Ratio of phospho-S6 RP to total S6 RP levels in the indicated cell lines normalized to MSCV-GFP–transduced control cells. Figure S3. GSI treatment reduces total cellular protein content (JPG, 53.5 KB) - Cells were treated with GSI (DAPT) or vehicle (DMSO) for 6 days, washed once with serum-free media, and resuspended in a small volume of PBS. The total of amount of protein was measured for an aliquot of the cell sample and normalized to the absolute number of cells in the aliquot. Error bars represent standard deviation. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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