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Blood, Vol. 109, Issue 5, 1945-1952, March 1, 2007
Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide–mediated vascular smooth muscle relaxation
Blood Isenberg et al.
109: 1945
Supplemental materials for: Isenberg et al, Vol 109, Issue 5, 1945-1952
Files in this Data Supplement:
- Figure S1. Disassembly of S1P-induced F actin by NO is attenuated in the presence of TSP1 (PDF, 668 KB) -
HAVSMCs were plated on glass chamber slides and incubated in basal medium with 0.1% BSA (A-B), 2.2 nM TSP1 (C-D), 100 nM S1P (E-H), or 10 µM 1H-1,2,4oxadiazole4,3-aquinoxalin-1-one (I-J) ± 10 µM DEA/NO for 5 minutes. Cells were then fixed, permeabilized, and stained with phalloidin to visualize F-actin. Photomicrographs representative of 3 separate experiments are presented.
- Figure S2. Random myocutaneous murine flap model (PDF, 1.58 MB) -
Random flaps 1 × 2 cm were developed sharply along the dorsal midline of C57B16 mice (WT and TSP1 null) as indicated and secured with several simple 5-0 nylon sutures. The undersurface of each flap was inspected following mobilization to ensure the absence of an identifiable axial vessel prior to flap inset.
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