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Blood, Vol. 109, Issue 9, 3706-3712, May 1, 2007
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells
Blood Zhu et al.
109: 3706
Supplemental materials for: Zhu et al, Vol 109, Issue 9, 3706-3712
Files in this Data Supplement:
- Figure S1. Addition of c-Kit ligand/IL-3/IL-6 to OB-LSC cocultures biases hematopoietic differentiation from B lymphopoiesis to myelopoiesis (JPG, 51.1 KB)
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FACS-sorted CD45.1+ Lin−Sca+ cells were cultured with CD45.2+ osteoblasts in the presence or absence of c-Kit ligand (100 ng/mL), IL-3 (6 ng/mL), and IL-6 (10 ng/mL) for 6 days. After culture, both adherent and suspended CD45.1+ cells were reanalyzed for the presence of (A) Sca+Kit+ (primitive myeloid) cells or (B) B220+ lymphocytes. Addition of c-KitL/IL-3/IL-6 shifted the cultures toward myelopoiesis and away from lymphopoiesis, in both adherent and nonadherent populations. Cultures initiated on OB monolayers with 104 LS cells produced 1.0 × 103 Sca+Kit+ cells and 1.4 × 103 B220+ cells (of 1.2 × 105 total adherent plus nonadherent hematopoietic cells). Cultures initiated on OB monolayers supplemented with c-KitL/IL-3/IL-6 produced 1.8 × 104 Sca+Kit+ cells and 0.8 × 103 B220+ cells (of 2.6 × 105 total hematopoietic cells).
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