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Blood, Vol. 109, Issue 10, 4406-4414, May 15, 2007
Growth factor independent 1B (Gfi1b) is an E2A target gene that modulates Gata3 in T-cell lymphomas
Blood Xu and Kee
109: 4406
Supplemental materials for: Xu and Kee, Vol 109, Issue 10, 4406-4414
Files in this Data Supplement:
- Figure S1. Differential regulation of Gfi1b during T-cell development (PDF, 24.1 KB) -
QPCR analysis of Gfi1b mRNA in sorted DN2 (Lin−CD117+CD25+), DN3 (Lin−CD117−CD25+), DN4 (Lin−CD117−CD25−), and DP (CD4+CD8+) thymocytes from wild-type mice.
- Figure S2. Gfi1b fails to bind to the conserved Gfi1b-binding sites in the Gata3 gene by EMSA (PDF, 42.5 KB) -
(A) Sequence of the oligos designed to cover potential Gfi1b sites in the Gata3 gene that are conserved between the mouse and human sequence. (B) In vitro–translated Gfi1b was incubated with each of the indicated double-stranded oligos (200× or 1000× excess) for 15 minutes prior to addition of radiolabeled consensus Gfi1b-binding oligo. Only the consensus Gfi1 oligo (200× excess) competed with the consensus oligo for binding to Gfi1b. In direct EMSA experiments Gfi1b showed detectable binding to the consensus oligo but not to Gfi1bBS-1, BS-2, or BS-3.
- Figure S3. Gata3 fails to bind to the conserved Gfi1b-binding sites in the Gfi1b gene by EMSA (PDF, 34.9 KB) -
(A) Sequence of the oligos designed to cover the potential Gata sites in the Gfi1b gene that are conserved between the mouse and human sequence. (B) 0531 cell extracts were incubated with radiolabeled T 2, Gata3 BS1, or Gata3 BS2 15 minutes after addition of anti-Gata3 antibody or 100× excess unlabeled Gata3 BS1 or Gata3 BS2.
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