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Blood, Vol. 109, Issue 3, 1289-1297, February 1, 2007
Skeleton-binding protein 1 functions at the parasitophorous vacuole membrane to traffic PfEMP1 to the Plasmodium falciparum–infected erythrocyte surface
Blood Maier et al.
109: 1289
Supplemental materials for: Maier et al, Vol 109, Issue 3, 1289-1297
Movies of z-stacks of immunofluorescence assay to assess trafficking of PfEMP1 in erythrocytes infected with parental CS2 and CS2 SBP1.
Files in this Data Supplement:
- Video S1. PfEMP1 is exproted to MC in CS2WT cells (AVI, 3.23 MB)
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Parental CS2 cells probed with anti-ATS antibody (green) showed typical PfEMP1 localization within the parasite and in punctate structures in the erythrocyte, which partially colocalized with the Maurer cleft marker PfMAHRP1 (red).
- Video S2. PfEMP1 accumulates in the PVM in CS2ΔSBP1 mutants (AVI, 4.32 MB)
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In CS2SBP1-infected erythrocytes, PfEMP1 (green) accumulated in patches around the parasite periphery, most likely in the PVM. Colocalization with PfMAHRP1 can only be observed in very few punctae around the parasite, whereas no PfEMP1 is exported and located on Maurer clefts (red). The movies show a rotational 3-dimensional image of serial sections of a representative cell in each case.
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