|
|
Blood, Vol. 109, Issue 11, 4825-4931, June 1, 2007
Interferon-producing killer dendritic cells (IKDCs) arise via a unique differentiation pathway from primitive c-kitHiCD62L+ lymphoid progenitors
Blood Welner et al.
109: 4825
Supplemental materials for: Welner et al, Vol 109, Issue 11, 4825-4831
Files in this Data Supplement:
- Figure S1. Model for direct comparison of progenitors (JPG, 99.8 KB)
-
Sorting schematic for collecting L-selectin+ progenitors (LSPs), early lymphoid progenitors (ELPs), common myeloid progenitors (CMPs), and common lymphoid progenitors (CLPs) from donor Thy1.1 congenic RAG-1/GFP reporter mice. Plots are representative of sorting experiments.
- Figure S2. Development of splenic IKDCs from progenitors (JPG, 134 KB)
-
(A) Representative plots of engraftment from intravenous transplant of sorted progenitors. Host mice received a sublethal dose of 6.5 Gy prior to injection of specified cells. Control mice that express CD45.1 and CD45.2 are seen for positive gating strategy. Contour plots show the gating scheme used for identifying IKDCs in the spleen. (B) LSPs, ELPs, CMPs, and CLPs were sorted as seen in Figure S1, and progenitor cells of each population were transferred to sublethally irradiated recipients. Flow cytometry analyses were then performed 16 days after transplantation. Absolute numbers of donor-derived pDCs (B220+CD19−CD11c+Ly6C+), IKDCs (B220+CD19−CD11c+Ly6C−NK1.1+), and NK cells (NK1.1+CD11c−) per spleen are given.
|
|
