|
|
Blood, Vol. 109, Issue 6, 2649-2656, March 15, 2007
In vivo dynamics of regulatory T-cell trafficking and survival predict effective strategies to control graft-versus-host disease following allogeneic transplantation
Blood Nguyen et al.
109: 2649
Supplemental materials for: Nguyen et al, Vol 109, Issue 6, 2649-2656
Files in this Data Supplement:
- Figure S1. Reduction of Tcon proliferation by Treg (PDF, 71.6 KB) -
Representative images demonstrating that the cotransfer of wild-type Treg at a dose ratio of 1:1 with alloreactive luc+ CD4+CD25− T cells (Tcon) led to the reduction in the expansion of Tcon without affecting their homing pattern. Tcon first localize and proliferate in secondary lymphoid organs with subsequent infiltration into peripheral tissues such as the skin.
- Figure S2. Prolonged Treg survival in vivo (PDF, 19.9 KB) -
Representative images day 91 following transplantation of donor T-cell–depleted bone marrow cells (TCD-BM) into lethally-irradiated animals that did or did not also receive allogeneic luc+ Treg on day 0 of transplantation. Prolonged survival of Treg is indicated by the persistent bioluminescence signal in the cervical lymph nodes and the skin (paws, ear, and tail).
|
|
