|
|
Blood, Vol. 109, Issue 9, 3963-3971, May 1, 2007
A leukemia fusion protein attenuates the spindle checkpoint and promotes aneuploidy
Blood Boyapati et al.
109: 3963
Supplemental materials for: Boyapati et al, Vol 109, Issue 9, 3963-3971
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 89.4 KB)
- Figure S1. The DNA-binding domain of AEtr is required for complete mitotic bypass (JPG, 34.9 KB)
-
(A) Lysates from K562 control or AEtrR174Q-transduced cells were used for anti-HA immunoblot to detect HA-AEtr R174Q expression. Ponceau S stain shows the relative protein loading in each lane. (B) The bar graph shows the mitotic index of vector control and AEtrR174Q cells after nocodazole or taxol treatment for 24 or 40 hours. The average of 2 independent experiments with multiple pools is shown. (C) The average percentages of vector control and AEtr cells that contained either 2N or 8N DNA contents following nocodazole treatment for 24 hours are shown for 3 independent experiments.
- Figure S2. Analysis of spindle checkpoint transcripts (JPG, 41.7 KB)
-
(A) RNA from vector control or AEtr cells treated with (+) and without (−) nocodazole was reverse transcribed, and cDNA was used in quantitative real-time RT-PCR analysis for BubR1, securin, cohesin, and GAPDH transcripts. The bar graph shows the relative levels of each mRNA after correcting for differences in GAPDH levels. The error bars show standard deviations. (B) Northern blot of securin mRNA. Total RNA from K562 vector control or AEtr cells treated with nocodazole for 18 hours was transferred to a membrane and hybridized with a radiolabeled human securin cDNA probe. Subsequent hybridization of the membrane with a human 18S RNA probe reflects the relative RNA loading in each lane.
|
|
