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Blood, Vol. 109, Issue 8, 3424-3431, April 15, 2007
Granulocyte colony-stimulating factor enhances bone tumor growth in mice in an osteoclast-dependent manner
Blood Hirbe et al.
109: 3424
Supplemental materials for: Hirbe et al, Vol 109, Issue 8, 3424-3431
Files in this Data Supplement:
- Figure S1. No Gr-1 high-expressing cells are observed in this macrophage culture system (JPG, 82.4 KB)
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Flow cytometry to assess expression level of Gr-1 was performed on bone marrow–derived macrophage cultures as well as on bone marrow enriched for neutrophils. No Gr-1 high-expressing cells were observed in the macrophage cultures, indicating an absence of neutrophils.
- Figure S2. Tumor growth in bone is increased following G-CSF administration in an intra-arterial model of bone metastasis (JPG, 214 KB)
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(A) Pretreatment schedule. Mice were administered 200 µg/kg G-CSF (n = 6) or vehicle (n = 6) daily for 8 days. On the fifth day of G-CSF or vehicle administration, 105 4T1-GFP-FL tumor cells were injected into the left ventricle. Ten days after tumor cells were injected, mice were killed and bones were isolated. Histomorphometric analysis demonstrated increased tumor burden in the G-CSF–administered animals (P = .008). Representative histology depicted here; M indicates marrow, T indicates tumor. (B) Posttreatment schedule. 4T1-GFP-FL tumor cells (105) were injected into the left ventricle. Mice were then randomized to receive G-CSF or vehicle and were administered 200 µg/kg G-CSF (n = 4) or vehicle (n = 4) daily for 8 days. Ten days after tumor cells were injected, mice were killed and bones were isolated. Histomorphometric analysis demonstrated increased tumor burden in the G-CSF–administered animals (P = .04). Representative histology depicted here; M indicates marrow, T indicates tumor.
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