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Blood, Vol. 109, Issue 7, 2797-2805, April 1, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef In vivo administration of lentiviral vectors triggers a type I interferon response that restricts hepatocyte gene transfer and promotes vector clearance Blood Brown et al. 109: 2797 Supplemental materials for: Brown et al, Vol 109, Issue 7, 2797-2805 Files in this Data Supplement: Figure S1. The innate immune response to LV is strain independent (PDF, 21 KB) - C57BL/6 mice were treated with 10 µg LV via intravenous injection, humanely killed at 4 hours after injection, and analyzed as in Figure 2. (A) Liver. (B) Spleen. Figure S2. The IFNαβ response mediates reduced transduction efficiency and plays a role in immune-mediated vector clearance (PDF, 52.7 KB) - (A) Confocal immunofluorescence analysis of spleen sections at the indicated time. Fixed frozen sections were costained with anti-GFP (green) antibodies to identify transduced cells. TO-PRO-3 (blue) was used to stain nuclei. Images are representative of 6 mice analyzed. Scale bars equal 75 µm. (B) Q-PCR analysis of LV DNA content within the spleen of normal 129sv (black) and IFNR–/– (gray) mice. All samples were normalized to murine -actin. Values are presented as the mean ± SEM (n = 6/group/time point). Asterisks indicate t-test significance value of 129sv compared with IFNR–/– mice (*P < .05; **P < .005). This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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