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Blood, Vol. 110, Issue 1, 313-322, July 1, 2007
Targeting autophagy augments the anticancer activity of the histone deacetylase inhibitor SAHA to overcome Bcr-Abl–mediated drug resistance
Blood Carew et al.
110: 313
Supplemental materials for: Carew et al, Vol 110, Issue 1, 313-322
Files in this Data Supplement:
- Figure S1. Effects of the CQ/SAHA and 3-MA/SAHA regimens on the cell-cycle distribution of Bcr-Abl–expressing cells (JPG, 35.5 KB)
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p210 Bcr-Abl–expressing Ba/F3 cells were treated with 25 µM CQ, 2 mM 3-MA, 1 µM SAHA, or the indicated combinations for 24 hours. Cells were then stained with propidium iodide and the percentages of cells in each phase of the cell cycle were quantified, based on DNA content using flow cytometry. Note that there were little effects on the cell-cycle distribution of CQ- or 3-MA–treated cells, whereas SAHA treatment led to more than a 2-fold decrease in the percentage of cells in S phase, and to a corresponding increase in the percentage of cells in G1. Notably, there were marked increases in the percentage of cells with sub-G0/G1 DNA content in CQ/SAHA- and 3-MA/SAHA–treated cells, which is indicative of apoptosis, and that these regimens effectively abolished cells in S phase. n = 3 (± SEM).
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