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Blood, Vol. 109, Issue 9, 4071-4079, May 1, 2007
New differentiation pathway for double-negative regulatory T cells that regulates the magnitude of immune responses
Blood Zhang et al.
109: 4071
Supplemental materials for: Zheng et al, Vol 109, Issue 9, 4071-4079
Files in this Data Supplement:
- Figure S1. CD4+ T-cell–converted DN T cells are NK1.1–, αβTCR+ and γδTCR– (JPG, 36.4 KB)
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The CD4+ converted DN T cells were   TCR+CD28+CD44+NK1.1–, which differed from thymus-derived   TCR+ DN T cells and from bone marrow–derived DN natural suppressor T cells that express NK1.1.25-27 (A) Isolated NK T cells from the spleen and lymph nodes of naive C57BL/6 mice were stained with antibodies to surface NK1.1 marker with CD4 T-cell–converted DN T cells side by side. (B) Isolated TCR+ DN T cells from the spleen and lymph nodes of naive C57BL/6 mice were stained with antibodies to surface or TCR markers with CD4 T-cell–converted DN T cells side by side. Indeed, we were able to isolate native TCR+CD28+CD44+NK1.1– DN T cells from the spleen and lymph nodes of naive C57BL/6 mice, and these DN T cells suppress alloantigen-triggered naive CD4+CD25– T-cell proliferation.
- Figure S2. αβTCR+CD28+CD44+NK1.1- DN T-cells from the spleen and lymph nodes of naïve C57BL/6 mice are suppressive T-cells (JPG, 38.7 KB)
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The isolated TCR+ DN T cells from the spleen and lymph nodes of naive C57BL/6 mice are TCR+CD28+CD44+NK1.1– and are capable of suppressing alloantigen-triggered naive CD4+CD25– T-cell proliferation in MLRs. (A) The converted CD4– T cells were stained with antibodies to indicated cell-surface markers. (B) DN T cells isolated from the spleen and lymph nodes of naive C57BL/6 mice suppress CFSE-labeled C57BL/6 CD4+CD25– T-cell proliferation triggered by mature DBA/2 DCs.
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