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Blood, Vol. 109, Issue 7, 2989-2998, April 1, 2007
Comprehensive characterization of IGHV3-21–expressing B-cell chronic lymphocytic leukemia: an Italian multicenter study
Blood Bomben et al.
109: 2989
Supplemental materials for: Bomben et al, Vol 109, Issue 7, 2989-2998
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 75.4 KB)
- Table S1. Genes differentially expressed in IGHV3-21 versus non–IGHV3-21 CLLs (PDF, 26.5 KB)
- Table S2. Representative gene ontology categories relatively enriched comparing IGHV3-21 versus non–IGHV3-21 CLLs (PDF, 115 KB)
- Table S3. Genes differentially expressed in homHCDR3–IGHV3-21 versus nonhomHCDR3–IGHV3-21 CLLs (PDF, 14.6 KB)
- Figure S1. HCDR3 clustering of CLL (PDF, 256 KB) -
HCDR3 sequences from a total of 349 cases of known IGHV rearrangement enrolled by different institutions were converted into aa sequences and clustered by means of the multiple sequence alignment software ClustalW (available at the EMBL-EBI, European Bioinformatics Institute website, www.ebi.ac.uk/clustalw/; accessed June 2006), which was used also to draw the dendrogram. The length of HCDR3 was computed between codons 95 and 102, as described by Kabat.21 Only IGHV rearrangements for IGHV3-21 CLL cases are indicated (arrows next to their corresponding branch). The enlargement in the upper part of the figure corresponds to the area of the tree containing homHCDR3–IGHV3-21 cases and the only case with non–IGHV3-21 rearrangement. The dotted line indicates the minimum distance that characterized the cluster containing the homHCDR3–IGHV3-21 cases. The inset represents IGHV gene family usage of the 349 reported cases. The histogram indicates the frequencies for each IGHV family, computed for the whole series (black columns) and according to the IGHV mutational status (UM, gray columns; M, white columns).
- Figure S2. Unsupervised analysis of CLL (PDF, 51.9 KB) -
Dendrogram showing the hierarchic clustering of 28 513 genes selected after a prefiltering process (Supplemental Materials, first paragraph) of gene expression data from 65 CLL cases. M and UM indicate the cases with mutated and unmutated IGHV mutational status, respectively, according to the standard 2% cutoff. Arrows indicate IGHV3-21 CLL cases.
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