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Blood, Vol. 109, Issue 8, 3284-3290, April 15, 2007
Structure-function analysis reveals discrete ß3 integrin inside-out and outside-in signaling pathways in platelets
Blood Zou et al.
109: 3284
Supplemental materials for: Zou et al, Vol 109, Issue 8, 3284-3290
Files in this Data Supplement:
- Figure S1. Platelet spreading on fibrinogen is independent of secondary ADP signaling (JPG, 101 KB)
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(A) Apyrase does not inhibit platelet spreading on fibrinogen. To determine if secondary signals such as secreted ADP are required for platelet spreading on fibrinogen, unstimulated wild-type platelets were exposed to immobilized fibrinogen with and without the ADP scavenger apyrase. Adherent platelets were visualized using actin staining with Alexa-Fluor 594 phalloidin (top), and the area occupied by adherent platelets was measured using the Image J program. P values are shown for comparison of values obtained with and without apyrase; n = 430 to 500 individual platelets per sample. (B) Inhibition of ADP-induced fibrinogen binding by apyrase. ADP-stimulated fibrinogen binding by wild-type platelets in the presence (green line) and absence (red line) of apyrase is shown.
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