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Blood, Vol. 109, Issue 10, 4127-4134, May 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef PF-4/CXCL4 and CXCL4L1 exhibit distinct subcellular localization and a differentially regulated mechanism of secretion Blood Lasagni et al. 109: 4127 Supplemental materials for: Lasagni et al, Vol 109, Issue 10, 4127-4134 Files in this Data Supplement: Figure S1. Specificity and sensitivity of primers and probes for CXCL4 and CXCL4L1 mRNA (JPG, 37.7 KB) - (A) Amplification of CXCL4 standard curve generated using 10-fold scalar dilutions of a known amount of a plasmid containing the CXCL4 amplicon using CXCL4- (black squares) or CXCL4L1-specific primers (white triangles). One representative experiment is depicted. (B) Amplification of CXCL4L1 standard curve generated using 10-fold scalar dilutions of a known amount of a plasmid containing the CXCL4L1 amplicon using CXCL4- (black squares) or CXCL4L1-specific primers (white triangles). One representative experiment is depicted. Figure S2. Absence of platelet contamination in T-cell and monocyte preparation (JPG, 18.9 KB) - GPIIb and GPIIIa mRNA levels in platelet, T-cell, and monocyte preparations, as assessed by quantitative RT-PCR. Mean values (± SEM) of triplicate determinations of 5 different donors are reported. Figure S3. Induction of CXCL4/CXCL4L1 mRNA expression by PMA in 3′5′ UTR-transfected HEK cells in comparison to HEK cells transfected with cDNAs without 3′5′ UTR (JPG, 44 KB) - mRNA expression of stable transfectants was assessed by quantitative RT-PCR after stimulation by 50 ng/mL PMA for 6 hours. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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