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Blood, Vol. 110, Issue 1, 211-219, July 1, 2007
Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper
Blood Hamdi et al.
110: 211
Supplemental materials for: Hamdi et al, Vol 110, Issue 1, 211-219
Files in this Data Supplement:
- Figure S1. CD45RO+ and CD25+ CD4+ T lymphocytes are required for the induction of Tregs by GILZ-transfected DCs (JPG, 55.5 KB)
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Control and GILZ-transfected DCs were pulsed with PPD and used to stimulate CD4+ T lymphocytes either unfractionated or depleted of CD45RO+, CD45RA+, or CD25+ cells. (a) Expression of IL-10 by CFSElo CD4+ T lymphocytes after stimulation with control (ν) or GILZ-transfected (-) DCs in a representative of 3 experiments. Control mAb staining obtained with GILZ-transfected DCs is shown as a dotted line. (b) CD4+ T lymphocytes were recovered at the end of the stimulation and added to CFSE-labeled PBMCs stimulated with PPD. Proliferation of CD4+ responders was analyzed in 3 independent experiments.
- Figure S2. Role of CD25+ T lymphocytes in the induction of Tregs by GILZ-transfected DCs (JPG, 34.3 KB)
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(a) Increasing numbers of CD25+CD4+ T lymphocytes were added to CD25–CD4+ T lymphocytes and to PPD-pulsed DCs transfected with either the control (□) or the GILZ-encoding (ν) vector. The fraction of IL-10–expressing CD4+ T lymphocytes was determined on day 7. Results (mean ± SEM) are from 3 experiments. (b) Purified CD4+CD25+ T lymphocytes were stimulated with PPD-pulsed DCs transfected with either the control (ν) or the GILZ-encoding (-) vector. IL-10 expression by CD4+ T lymphocytes was determined 7 days later.
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