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Blood, Vol. 111, Issue 6, 3266-3275, March 15, 2008
Costimulatory blockade of CD154-CD40 in combination with T-cell lymphodepletion results in prevention of allogeneic sensitization
Blood Xu et al.
111: 3266
Supplemental materials for Xu et al
Files in this Data Supplement:
- Figure S1. The effect of anti-ICOS or anti-OX40L on allosensitization. (PDF, 59.2 KB) -
BALB/c skin grafts were placed on B6 mice on day 0. Recipient B6 mice were treated with anti-ICOS or anti-OX40L mAb alone, or in combination with anti-  -TCR mAb. (A) Survival of BALB/c skin graft in B6 mice treated with anti-ICOS alone, anti-OX40L mAb alone, or in combination with anti- -TCR mAb. Recipients without mAb treatment served as controls. (B) Anti-donor antibody production after treatment with anti-ICOS alone, anti-OX40L mAb alone, or in combination with anti--TCR mAb. Naïve and sensitized B6 mice served as controls. Sera were collected 4 weeks after skin grafting and analyzed in FCXM assay. Antibody titers were reported as MFI. (C) BMT was performed 5-7 weeks after the treatment with anti-ICOS or anti-OX40L mAb alone, or in combination with anti- -TCR mAb at the time of initial skin grafting. Naïve and sensitized B6 mice served as controls. Recipients were conditioned with 950 cGy TBI and transplanted with 15 × 106 untreated BALB/c donor BMC via lateral tail vein injection 4-6 hours after irradiation. Animals were analyzed for engraftment using flow cytometric analysis 4 weeks after BMT by determining donor-derived PBL.
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