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Blood, Vol. 109, Issue 10, 4191-4199, May 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef B-cell development fails in the absence of the Pbx1 proto-oncogene Blood Sanyal et al. 109: 4191 Supplemental materials for: Sanyal et al, Vol 109, Issue 10, 4191-4199 Files in this Data Supplement: Table S1. Antibodies used for flow cytometric analysis (PDF, 30.2 KB) Figure S1. Configurations of Pbx1 alleles (JPG, 35.1 KB) - Gene targeting of the Pbx1 wild-type (wt) allele (top line) resulted in the insertion of a LoxP-PGK-hygromycin-GFP fusion cassette at the unique BstB1 site downstream of Pbx1 exon 3 and placement of a LoxP site upstream of exon 3 at the BamH1 site (second line). The floxed exon 3 was excised by in vitro Cre recombination to produce an inactivated Pbx1 allele (third line). Correctly targeted clones were identified by the presence of a 16-kb Ssp1 fragment on Southern blot analysis (3′ probe). The Pbx1 null allele generated by insertion of a PGK-neo cassette into exon 3 (bottom line) was described previously.1 Figure S2. Lentiviral vector for rescue of Pbx1b expression in Pbx1−/− ES cells (JPG, 9.02 KB) - Figure S3. Cellular architecture of the spleens from mice given fetal liver transplants (JPG, 106 KB) - Hematoxylin-and-eosin preparations of the spleens were analyzed for cellular architecture (left panels). A portion of the spleen was processed for cryosection and stained with Texas red–conjugated anti-B220 monoclonal antibody (middle panels). Single-cell preparations of the spleens were stained with fluorochrome-conjugated anti-B220, IgM, and IgD antibodies and analyzed by flow cytometry (right panels). REFERENCE 1. Selleri L, Depew MJ, Jacobs Y, et al. Requirement for Pbx1 in skeletal patterning and programming chondrocyte proliferation and differentiation. Development. 2001;128:3543-3557. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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