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Blood, Vol. 109, Issue 9, 3687-3696, May 1, 2007
HOXA10 is a critical regulator for hematopoietic stem cells and erythroid/megakaryocyte development
Blood Magnusson et al.
109: 3687
Supplemental materials for: Magnusson et al, Vol 109, Issue 9, 3687-3696
Files in this Data Supplement:
- Table S1. Lineage distribution in peripheral blood 16 weeks after transplantation of in vitro–cultured rtTA-HA10 LSK cells (PDF, 46.9 KB)
- Figure S1. Increasing concentrations of HOXA10 promote differentiation of Mac1+Gr– cells (PDF, 90 KB) -
Graded expression of HOXA10 in BM cells cultured in vitro in IL-3, IL-6, and SCF promotes the development of Gr+ and Mac1+ cells. An increase in myeloid differentiation is seen with increasing concentrations of HOXA10, particularly of the Mac1+Gr– population.
- Figure S2. Anemia and prominent splenomegaly develops after HOXA10 induction in vivo (PDF, 132 KB) -
Splenomegaly develops in wt recipients transplanted with rtTA-A10 bone marrow 4 weeks after HOXA10 induction. The increase in spleen size is correlated with both the hemoglobin and the hematocrit values over time. Correlation curve was created using Microsoft Excel Trendline (Microsoft, Redman, WA).
- Figure S3. High levels of HOXA10 in vivo perturb T-cell development and promote myeloid differentiation (PDF, 81.4 KB) -
rtTA-HA10 bone marrow cells (Ly5.2/5.2) were transplanted with WT support bone marrow (Ly5.1/5.1) at a ratio of 1:1. (A) Representative FACS analysis showing expression of CD4 andCD8 from an induced and a noninduced recipient mouse 16 weeks after transplantation. (B) FACS analysis for lineage distribution of peripheral blood (PB) 16 weeks after HOXA10 induction. Black bar represents induced recipients with rtTA-HA10 bone marrow cells, and gray bars represent recipients with uninduced rtTA-HA10 bone marrow cells; (n=4) / P < .05.
- Figure S4. 5′ RACE sequences (PDF, 12 KB) -
Sequences derived from the 5′ RACE assay using cDNA from Lin– bone marrow cells. These sequences were then used for finding the transcriptional start site by BLAST search for the identified product using the NCBI database.
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