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Blood, Vol. 110, Issue 8, 2955-2964, October 15, 2007
Critical role of FLT3 ligand in IL-7 receptor–independent T lymphopoiesis and regulation of lymphoid-primed multipotent progenitors
Blood Sitnicka et al.
110: 2955
Supplemental materials for: Sitnicka et al, Vol 110, Issue 8, 2955-2964.
Files in this Data Supplement:
- Document S1. Supplementary Materials and Methods (PDF, 33.6 KB)
- Figure S1. Loss of early thymic progenitors in adult mice double deficient in Fl and Il7r expression (PDF, 108 KB) -
Thymocytes from 8- to 10-week-old wild-type (WT), Fl-/-, Il7r-/-, and Fl-/-Il7r-/-mice were stained with antibodies against CD3, CD4, CD8, B220, CD19, TCR , Mac-1, Gr-1, Ter119, CD44, CD25, and CD45.2. (A) Representative plots of CD45 expression within DN4 thymic progenitors (gated as CD3-CD4-CD8-CD44-Ter119-Mac-1-B220-CD19-CD25-) of 8- to 10-week-old WT and Fl-/-Il7r-/-mice. (B) Representative plots of TCR expression within CD8+ thymocytes (gated on viable CD4-CD8+thymocytes). Immature, single-positive (ISP) thymocytes indicated by a box.
- Figure S2. Age-dependent reduction in thymic cellularity in mice double deficient in Fl and Il7r expression (PDF, 54 KB) -
Thymuses were isolated from 17- to 18-day fetuses and 2- and 5-week-old WT, Il7r–/–, Fl–/–, and Fl–/–Il7r–/– mice and cellularities were enumerated. (A) Total thymic cellularities in day 17 to 18 fetuses. Mean (SD) values from 7 to 16 fetuses from at least 2 different litters. (B) Mean (SD) values from 9 to 26 two-week-old mice. (C) Mean (SD) values from 6 to 11 five-week-old mice.*P < .01 comparing Il7r-/- and Fl-/-Il7r-/- mice.
- Figure S3. Reductions in thymic progenitors and peripheral T cells in mice double deficient in Fl and Il7r expression (PDF, 73 KB) -
Total thymic and peripheral-blood cellularity was determined in 5-week-old WT, Fl-/-, Il7r-/-, and Fl-/-Il7r-/- mice. (A) Total number of DN1-DN4 populations per thymus. (B) Total number of double-positive (DP) and single-positive (SP) cells per thymus. (C) Total number of CD4 and CD8 T cells in peripheral blood. All data represent mean (SD) values of 5 to 7 mice. For double-negative (DN) analysis, Il7r-/- and Fl-/-Il7r-/- thymuses were pooled. 0 indicates undetectable levels. *P < .01 comparing Il7r-/- and Fl-/-Il7r-/- mice; **P < .01 comparing WT and Fl-/- mice.
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