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Blood, Vol. 109, Issue 10, 4487-4493, May 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils Blood von Vietinghoff et al. 109: 4487 Supplementary materials for: von Vietinghoff et al, Vol 109, Issue 10, 4487-4493 Files in this Data Supplement: Figure S1. Confocal microscopy of live neutrophils showed colocalization of PR3- and NB1-stained spots on the plasma membrane (PDF, 117 KB) Figure S2. Transfection of a NB1 expression plasmid into HL60 cells (PDF, 30.7 KB) - Transfection of an NB1 expression plasmid into HL60 cells resulted in an increase in binding of exogenous PR3 that was not observed in control plasmid-transfected cells. Control- or NB1-transfected HL60 cells were loaded with human PR3 (5 µg/mL) for 120 minutes. Flow cytometry was performed after staining with isotype control antibody, anti-NB1 ab, and anti-PR3 ab, respectively. Dot plots and percentages from a typical experiment of 3 are provided. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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