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Blood, Vol. 109, Issue 10, 4360-4367, May 15, 2007
Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity
Blood Chen et al.
109: 4360
Supplemental materials for: Chen et al, Vol 109, Issue 10, 4360-4367
Files in this Data Supplement:
- Figure S1. IgG deposit in the kidney of recipients injected with Bim−/− DCs (PDF, 18.3 KB) -
WT or Bim−/− BMDCs were injected into syngenic C57BL/6 recipient mice intraperitoneally, followed by injection of LPS intraperitoneally (30 µg/mouse) 24 hours later. Kidneys were collected 2 weeks later, and frozen sections were stained with FITC-conjugated goat anti–mouse IgG (Sigma) and analyzed under a fluorescence microscope. IgG deposit was detectable in approximately 50% of recipients injected with Bim−/− DCs but not WT DCs.
- Figure S2. Promotion of B-cell proliferation by DCs (PDF, 15 KB) -
(A) CD11c+ WT and Bim−/− BMDCs were stimulated with 100 ng/mL LPS for 16 hours. Different numbers of DCs were added to B cells (2 × 105/well) in the presence of 1 µg/mL F(ab)′2 anti-IgM (Jackson Immunoresearch, West Grove, PA). The cells were cultured for 2 days and pulsed with 1 µCi (0.037 MBq)/well 3H-thymidine during the last 8 hours to determine 3H-thymidine incorporation. (B) CD11c+ WT BMDCs were stimulated with 100 ng/mL LPS for 16 hours. DCs were either untreated or irradiated (60 Gy) and then added to B cells (2 × 105/well) in the presence of 1 µg/mL F(ab)′2 anti-IgM. Cell proliferation was determined as in panel A. Data (mean ± SEM) are representative of 3 independent experiments.
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