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Blood, Vol. 110, Issue 2, 568-577, July 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef HLA-G expression defines a novel regulatory T-cell subset present in human peripheral blood and sites of inflammation Blood Feger et al. 110: 568 Supplemental materials for Feger et al, Vol 110, Issue 2, 568-577 Files in this Data Supplement: Document 1. Supplemental materials and methods (PDF, 21.1 KB) Figure S1. CD8 HLA-G+ T cells showed a more naive phenotype compared to their negative counterparts (JPG, 58.8 KB) - Gated CD4 HLA-G+/− (top) and CD8 HLA-G+/− (bottom) were counterstained with CCR7 and CD45RA and the distribution of naive/memory cells was calculated. A representative staining is shown. Figure S2. CD8 HLA-Gpos T cells were hypoproliferative and had suppressor cell activity (JPG, 39.3 KB) - (A) CD8 HLA-G− (black bars) and CD8 HLA-G+ (white bars) T cells were stimulated with anti-CD3/CD28 beads or with allogeneic PBMC (allo MLR). Proliferation was determined by 3H incorporation after 4 days. A representative experiment is shown. (B) CD8 HLA-G+ T cells were titrated into CFDA-SE labeled CD8 HLA-G− T cells in the presence of allogeneic PBMC and soluble anti-CD3. Cell proliferation was measured by fluorescent-activated cell sorting (FACS) analysis after 4 days in culture. A representative experiment is shown. Figure S3. No difference in the distribution of CD8 HLA-G+ T cells in CSF and blood of NIND, OIND and MS patients (JPG, 82.2 KB) - (A) FACS analysis of paired blood and CSF specimens from patients with neuroinflammatory disease were performed. Gates for CD8+ lymphocytes were set on CD8/FSC dot plots respectively, and the percentage of HLA-G+ cells was assessed in comparison to negative control. A representative staining is shown. (B) Summary of paired samples of blood and CSF specimens for CD8 HLA-G+ T cells from NIND (left), OIND (middle), and MS patients (right). Mean with SEM is shown in the upper panel. The lower panel shows paired percentages for each patient. (C) Comparison of CD8 HLA-G+ T cell percentages of MS patients in acute and stable phase of the disease. Mean with SEM is shown in upper panel; lower panel shows single patient pairing. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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