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Blood, Vol. 109, Issue 12, 5463-5472, June 15, 2007
ß-Catenin stabilization stalls the transition from double-positive to single-positive stage and predisposes thymocytes to malignant transformation
Blood Guo et al.
109: 5463
Supplemental materials for: Guo et al, Vol 109, Issue 12, 5463-5472
Files in this Data Supplement:
- Table S1. Expression profile of CD4Cre-Ctnnbex3 compared to LckCre thymocytes (PDF, 89.3 KB) -
Genes were filtered for variation across samples: 0.30|<|Standard deviation/Mean|<|10.00, P Call%| ≥ |50%, obtaining 4913 genes out of 45|101 genes. Genes in the table were selected for expression changes of more than 3-fold between LckCre and CD4Cre-Ctnnbex3 thymocytes (100 genes in total).
- Table S2. Expression differences between CD4Cre-Ctnnbex3 thymocytes and the resulting lymhpomas (PDF, 170 KB) -
Genes were filtered for variation across samples: 0.30|<|Standard deviation/Mean|<|10.00, P Call%| ≥ |50%, obtaining 4913 genes out of 45|101 genes. Genes in the table were selected for expression changes of more than 3-fold between CD4Cre-Ctnnbex3 pretransformed thymocytes and derived lymphomas (244 genes in total).
- Figure S1. β-Catenin stabilization induces thymic lymphomas (PDF, 756 KB) -
Hematoxylin/eosin staining of sections from a CD4Cre-Ctnnbex3 mouse with lymphoma. (Left) 20× magnification of the lymphoma, showing also the heart and lungs. (Right) 400× amplification of the same lymphoma. Arrows point to dense dividing nuclei. Images are representative of lymphomas in five CD4Cre-Ctnnbex3 and four LckCre-Ctnnbex3 mice.
- Figure S2. Hierarchical map of genes differentially expressed in control versus CD4Cre-Ctnnbex3 thymocytes and CD4Cre-Ctnnbex3 derived lymphomas (PDF, 54.6 KB) -
Gene expression values are normalized and color-coded, as indicated by the scale beneath the graph. Red indicates up-regulation while blue indicates down-regulation. Genes validated by independent approaches are marked in red. Genes related to T cell development are in black. Only genes showing more than 3 fold difference between the control and CD4Cre-Ctnnbex3 or CD4Cre-Ctnnbex3- and CD4Cre-Ctnnbex3-derived lymphomas are shown. (A) Illustrates the relative expression of genes in CD4Cre-Ctnnbex3 thymocytes as compared to LckCre controls. This comparison selected 100 genes (Table S1). (B) Illustrates the relative expression of genes in CD4Cre-Ctnnbex3 thymocytes compared to CD4Cre-Ctnnbex3 lymphomas. This comparison selected 244 genes (Table S2). Each column represents an independent RNA preparation from the indicated mice.
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