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Blood, Vol. 110, Issue 1, 242-250, July 1, 2007
Crosstalk between RANKL and Fas signaling in dendritic cells controls immune tolerance
Blood Izawa et al.
110: 242
Supplemental materials for: Izawa et al, Volume 110, Issue 1, 242-250
Files in this Data Supplement:
- Figure S1. Effect of RANK siRNA on Fas expression and apoptosis through RANKL and Fas in DCs (PDF, 263 KB) -
(A) Experimental protocol of RANK siRNA transfection using BMDCs from MRL+/+ and MRL/lpr mice is shown. (B) RANK expression on BM cells and RANK siRNA-transfected BMDCs from MRL+/+ and MRL/lpr mice was analyzed by flow cytometric analysis. Irrelevant oligonucleotides were used as control. Data are representative of 3 independent experiments. (C) RANK expression of RANK siRNA-transfected BMDCs from MRL+/+ and MRL/lpr mice was detected by immunoblot. Irrelevant oligonucleotides were used as control. GAPDH was used as a control for loading. Results are representative of 3 independent experiments. (D) Fas expression on RANK siRNA-transfected BMDCs from MRL+/+ and MRL/lpr mice was analyzed after the stimulation of RANKL. Irrelevant oligonucleotides were used as control. Numbers above horizontal lines indicate percentage of Fas+ cells. Data are representative of 3 independent experiments normalized to the control values. (E) Inhibitory effect of RANK siRNA on RANKL-induced apoptosis of BMDCs from MRL+/+ and MRL/lpr mice was observed. Apoptotic cells were detected by flow cytometric analysis. Numbers above horizontal lines indicate percentage of annexin-V+ cells. Results are representative of 3 independent experiments.
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