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Blood, Vol. 109, Issue 12, 5481-5490, June 15, 2007
Dynamic regulation of Gata factor levels is more important than their identity
Blood Ferreira et al.
109: 5481
Supplemental materials for: Ferreira et al, Vol 109, Issue 12, 5481-5490
Files in this Data Supplement:
- Figure S1. Potential translational regulatory motifs in the Gata2 1G 5′UTR (JPG, 34.5 KB)
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(A) The 5′ untranslated region (UTR) encoded by the Gata2 1G exon contains 2 out of frame AUG codons embedded in consensus translation initiation motifs.55 Translation of upstream open reading frames 1 and 2 (uORF1 and uORF2) would result in short peptides of 22 and 63 amino acids (aa), respectively. uORF2 overlaps the translation initiation codon of Gata2 (Gata2 ORF). (B) Putative secondary structure of the Gata2 1G 5′UTR calculated by the Mfold computer program.57 The 1G 5′UTR may adopt a Y-shaped secondary structure embedding the AUG codons of the uORFs and the canonical Gata2 start codon. The two most prominent loops in this structure are potential binding sites for poly(rC) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB), proteins known to be involved in the control of translation initiation.56 Red bars: GC base pairs; blue bars: AU/GU/AC base pairs; numbers indicate nucleotide positions. AUG codons of uORF1, uORF2, and Gata2 ORF are indicated. Green lines highlight potential PCBP1/PTB binding sites.
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