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Blood, Vol. 110, Issue 6, 2013-2019, September 15, 2007
An NKT-mediated autologous vaccine generates CD4 T-cell–dependent potent antilymphoma immunity
Blood Chung et al.
110: 2013
Supplemental materials for Chung et al, Vol. 110, Issue 6, 2013-2019
Files in this Data Supplement:
- Figure S1. Comparison of antitumor effects between A20/αGC and A20 plus αGC vaccination (PDF, 24.9 KB) -
BALB/c mice were vaccinated with A20/ GC or A20/veh plus intraperitoneal injection of GC (1 mg/mL) at day -7 (A) (n=5 per group) or at day 5 (B) (n=8 per group). Nonvaccinated mice were used as control (Nil). On day 0, all mice received 106 live A20 via intravenous route, and the survival was monitored. P values were calculated in comparison with nonvaccinated group by Kaplan-Meier analysis.
- Figure S2. Loading of αGC does not induce apoptosis in A20 in vitro (PDF, 52.3 KB) -
(A,B), A20 cells were cocultured with GC (1 µg/mL) or the same volume of vehicle (5 µL/mL of 0.5 % polysorbate) for 24 or 48 hours. (A) Cells were stained with FITC-conjugated annexin V according to manufacturer’s instruction. (B) Cells were permeabilized and intracellular active caspase-3 were stained with biotin-conjugated anti-active caspase-3 Ab followed by PE-conjugated-avidin staining. (C) A20 cells were cocultured with overnight with vehicle (A20/veh) or 1 µg/mL of GC (A20/GC) before being labeled with 1 µM DDAO SE (Molecular Probes, Eugene, OR). The labeled A20 cells were cocultured with BALB/c splenocytes (107/mL) for 24 or 48 hours. FITC-conjugated Annexin V and 7-AAD staining was performed and DDAO SE-positive cells were gated and analyzed.
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