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Blood, Vol. 110, Issue 8, 3039-3048, October 15, 2007
HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA–forming microsatellite
Blood Bayele et al.
110: 3039
Supplemental materials for: Bayele et al, Vol. 110, Issue 8, 3039-3048.
Files in this Data Supplement:
- Table S1. Oligonucleotides for HRE enhancer constructs (PDF, 14.7 KB). -
NheI and XhoI half-sites are underlined.
- Table S2. Sequences of allele-specific microsatellites and a common regulatory SNP used in interchange reactions by site-directed mutagenesis (PDF, 48.1 KB). -
Note that due to differences in transcription start site assignment, the regulatory SNP-209T in this study is the same as SNP-237T.1,2 This SNP was mutated to the more common -209C. Microsatellite sequences for alleles 1, 2, 6, and 7 as well as the mutant microsatellite (GT)22 were used for interchange reactions using allele 3 (SLC11A1-luc) as template.
- Table S3. Allele-specific duplex microsatellites used for mobility shift assays (PDF, 19.8 KB). -
Complementary phosphorylated oligonucleotides were synthesized (MWG Biotech). Mutant microsatellite oligonucleotide (GT)22 was generated by mutating the AC dinucleotides in the two HREs to GT.
REFERENCES
1. Zaahl MG, Robson KJH, Warnich L, Kotze MJ. Differential expression of the 5′-(GT)n repeat in the SLC11A1 gene: opposite allelic effect in the presence of the –237C→T polymorphism. Blood Cells Mol Dis. 2004;33:45–50.
2. Lewis LA., Victor TC, Helden EG, et al. Identification of a C to T mutation at position –236 bp in the human NRAMP1 promoter. Immunogenet. 1996;44:309-311.
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