|
|
Blood, Vol. 110, Issue 4, 1251-1261, August 15, 2007
The C-MYB locus is involved in chromosomal translocation and genomic duplications in human T-cell acute leukemia (T-ALL), the translocation defining a new T-ALL subtype in very young children
Blood Clappier et al.
110: 1251
Supplemental materials for Clappier et al, Vol 110, Issue 4, 1251-1261
Files in this Data Supplement:
- Table S1. List of genes used for the profiling analysis (PDF, 27.3 KB) -
This list is highly significantly (P < 10-7) enriched in genes characterized by gene ontology (GO) terms including “cell cycle” and “cell proliferation,” and more specifically in GO terms related to “mitosis” (M phase of mitotic cycle, nuclear division, cytokinesis, see also Table S2).
- Table S2. Main gene ontology terms linked to the genes highly expressed in the TCRB-MYB T-ALL and T-cell lines cluster (XLS, 41 KB)
- Figure S1. Molecular characterization of the T-ALL t(6;7) cases T142 and UPN5846 (JPG, 116 KB)
-
- Figure S2. Absence of reported copy number variation in the 6q23.3 C-MYB locus in the database of genomic variants (JPG, 137 KB)
-
- Figure S3. Expression analysis of the genes at the 6q23.3 rearranged locus in the T-ALL cases (JPG, 76.7 KB)
-
- Figure S4. Strategy and data for analysis of C-MYB alternative expression (JPG, 171 KB)
-
(A) Scheme of the human C-MYB gene and main transcripts, and PCR strategy for analysis of C-MYB alternative transcripts. A classical nomenclature has been used in order to keep the Exon 9, 9A, and Exon 10 names. Real-time quantitative polymerase chain reaction(RQ-PCR) systems are shown in red, other primer for real-time polymerase chain reaction (RT-PCR) in black. (B) List of primers and probes, and sequences, as used for C-MYB RT-PCR analysis. (C) RQ-PCR expression data of main (S1) and variant long (S2) C-MYB transcripts in t(6;7), MYBdup, other T-ALLs, and normal controls. (D) RQ-PCR data for analysis of the use of promoter upstream of Exon 1 (P1), and variant promoter upstream of Exon 2 (P2) in t(6;7), MYBdup, other T-ALLs, and normal controls. (E) Search for C-MYB alternative short exons between Exon 8 / Exon 9, and Exon 9 / Exon10, according to rare mRNA sequences in Genebank. Representative T-ALL cases with t(6;7), MYBdup, other T-ALLs, and normal controls were analyzed; data from 4 T-cell lines are shown. (F) Immunoblot analysis of C-MYB expression in a panel of primary T-ALL samples and T-cell lines (Anti-Myb, clone 1-1, Upstate; loading control using anti-gamma tubulin ab11321, Abcam).
- Figure S5. Unsupervised hierarchical classification of T-ALL samples using large-scale gene expression data (JPG, 91.9 KB)
-
- Figure S6. Immunophenotype data of the TCRB-MYB cases (JPG, 44.3 KB)
-
| |
