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Blood, Vol. 110, Issue 1, 441-449, July 1, 2007
Keratinocyte growth factor augments immune reconstitution after autologous hematopoietic progenitor cell transplantation in rhesus macaques.
Blood Seggewiss et al.
110: 441
Supplemental materials for: Seggewiss et al, Volume 110, Issue 1, 441-449
Files in this Data Supplement:
- Table S1. Transplantation characteristics (PDF, 22.6 KB)
- Table S2. Antibodies for phenotyping T cells (PDF, 54.9 KB)
- Figure S1. Reconstitution of T cells in blood after transplantation (JPG, 78.7 KB)
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(A) Frequencies of CD3+ T cells in peripheral blood before and after transplantation; animals given multiple doses of KGF are indicated with blue symbols, those given a single dose of KGF with green symbols, and control animals with red symbols. (B,C) Frequencies of naive CD4+ and CD8+ T cells in peripheral blood over time. Data are displayed as median and interquartile range. For the 2 multiple-dose animals, only the median is plotted, as an IQR would be statistically unreliable.
- Figure S2. Thymic histology (JPG, 171 KB)
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H & E stains of the thymus of the animals at necropsy 12 to 14 months after transplantation. The column on the left indicates that thymi scored as grade 1 have well-preserved architecture with well-defined cortex and medulla, and no atrophic changes. The middle column indicates that grade 2 thymi have some degree of atrophic changes consisting of fat replacement of lobules and preservation of cortex and medulla, and islets of cell dense tissue containing many thymocytes. The right column indicates that grade 3 thymi have marked atrophic changes and cystic degeneration. All photos were taken with an Olympus BX41 microscope equipped with a 4×/0.13 UPlanFl objective lens.
- Figure S3. Reconstitution of T cells according to thymus histology (JPG, 103 KB)
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The reconstitution of naive CD4+ (A) and naive CD8+ (B) T cells and reconstitution of overall CD4+ (C) and CD8+ (D) T-cell numbers in the peripheral-blood compartment in animals with well-preserved grade 1 (blue symbols), intermediate grade 2 (green symbols), and atrophic grade 3 (red symbols) thymi. Data are displayed as median and interquartile range. For the 2 multiple dose animals, only median is plotted, as an IQR would be statistically unreliable.
- Figure S4. Thymus histology and TCR repertoire diversity (PDF, 38.2 KB)
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The graph shows the ranking of TCR repertoire diversity in 10 animals (from 1, most diverse to 11, least diverse) based on the product of the skewness and kurtosis of their TCR spectratype. This is compared with the 3 groups of thymic architecture defined as histologically comprising well-preserved (grade 1), intermediate (grade 2), and atrophic (grade 3) thymi.
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