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Blood, Vol. 110, Issue 2, 501-508, July 15, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Bovine apolipoprotein B-100 is a dominant immunogen in therapeutic cell populations cultured in fetal calf serum in mice and humans Blood Sakamoto et al. 110: 501 Supplemental materials for Sakamoto et al, Vol. 110, Issue 2, 501-508 Kinetic analysis of cell surface—bound bovine apoB-100 BW5147.3 cells cultured in 10% FCS—DMEM were washed three times with serum-free DMEM. BW5147.3 cells (106) were cultured in 2 mL serum-free DMEM in a 24-well plate. At 0, 2, 4, 8, and 12 hour time points, cells were collected, gently washed once with PBS, and stained with FITC-conjugated 3E8.1 or isotype-matched control antibody. FACS analysis was immediately performed. Propidium iodide PBS (10 µg/mL) was added to each tube to provide for a live gate. Files in this Data Supplement: Figure S1. Kinetics of cell surface—bound bovine apoB-100 in serum-free medium (JPG, 78.8 KB) - (A) BW cells cultured in serum free DMEM were stained with 3E8.1-FITC at the onset of the experiment (dark solid line) or two hours later (dotted line). The thin solid line is the isotype matched antibody profile. (B) BW cells cultured in serum free DMEM were stained with 3E8.1-FITC at the various time points indicated. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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