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Blood, Vol. 110, Issue 6, 1730-1738, September 15, 2007
p52Shc is required for CXCR4-dependent signaling and chemotaxis in T cells
Blood Patrussi et al.
110: 1730
Supplemental materials for Patrussi et al, Vol. 110, Issue 6, 1730-1738
Files in this Data Supplement:
- Figure S1. Time course analysis of CXCR4-dependent protein phosphorylation (JPG, 29.4 KB)
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(A) Phosphorimager analysis (left) and quantification (right) of enolase phosphorylation in in vitro kinase assays of Lck-specific immunoprecipitates of Jurkat cells, activated for the indicated times with SDF-1 . The results are presented as relative phosphorylation in SDF-1-treated versus untreated cells (the latter taken as 100%, n=2). Error bars are a SD. (B,F) Immunoblot analysis with (B) anti-phospho-ZAP-70 or (F) antiphospho-Erk1/2 antibodies of lysates of Jurkat cells, either untreated or treated for the indicated times with SDF-1. (C-E) Antiphosphotyrosine immunoblot analysis of (C) LAT-, (D) Vav-, or (E) Itk-specific immunoprecipitates from lysates of Jurkat cells, either untreated or treated for the indicated times with SDF-1. Control blots of the stripped filters are shown below. The migration of molecular mass markers is indicated.
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