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Blood, Vol. 110, Issue 2, 719-726, July 15, 2007
Haploinsufficiency of EGR1, a candidate gene in the del(5q), leads to the development of myeloid disorders
Blood Joslin et al.
110: 719
Supplemental materials for Joslin et al, Vol 110, Issue 2, 719-726
Files in this Data Supplement:
- Table S1. Mice analyzed using single strand conformation polymorphism analysis (PDF, 200 KB)
- Table S2. Fluorescence in situ hybridization analysis (PDF, 52.6 KB) -
Cells were hybridized with a biotin-labeled P1 artificial chromosome (PAC) probe (98D9, detected with fluorescein isothiocyanate FITC—conjugated avidin) from mouse chromosome 15 containing the Myc gene. Interphase cells (100-200) were scored, and the distribution of cells with 0, 1, 2, 3, or 4 signals is shown. The mean + 3 SD for three signals is 7.76. The cut-off value for trisomy 15 is 8%.
- Table S3. Primers used in this study (PDF, 49.7 KB)
- Figure S1. Complete blood counts of WT, Egr1+/−, and Egr1−/− mice (JPG, 76.5 KB)
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Complete blood counts were performed every 6 weeks starting at 8 weeks of age. Egr1−/− mice display mildly elevated WBC counts, as a result of elevated lymphocyte counts, as compared to wild-type and heterozygous mice. WBC indicates white blood cell; LY%, percent of leukocytes that are lymphocytes; NE%, percent of leukocytes that are neutrophils; MO%, percent of leukocytes that are monocytes; EO%, percent of leukocytes that are eosinophils; BA%, percent of leukocytes that are basophils; RBC, red blood cell counts; Hb, mass or weight of hemoglobin in the specified volume of blood; HCT, hematocrit or relative volume of erythrocytes; MCV, mean corpuscular volume (average volume of individual erythrocytes); RDW, red cell distribution width; PLT, number of platelets; MPV, mean platelet volume. Values for wild-type mice are shown in blue, those for Egr1+/− are shown in red, and those for Egr1−/− are shown in green.
- Figure S2. In vitro differentiation assays (JPG, 67.5 KB)
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Bone marrow was collected at 6-month intervals, up to 18 months of age, for in vitro progenitor colony growth assays. For CFU-E assays, 2 × 105 total bone marrow cells were plated in duplicate in Methocult media containing hEpo (Stem Cell Technologies). For all other assays, 2 × 104 total bone marrow cells were plated in duplicate in Methocult media containing mSCF, mIL-3, hIL-6, and hEpo (Stem Cell Technologies). The average colony numbers for 3 mice for each time point is graphed. *P=0.046, **P=0.009.
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