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Blood, Vol. 110, Issue 10, 3582-3590, November 15, 2007
The Shc-binding site of the ßc subunit of the GM-CSF/IL-3/IL-5 receptors is a negative regulator of hematopoiesis
Blood Ramshaw et al.
110: 3582
Supplemental materials for: Ramshaw et al
Files in this Data Supplement:
- Figure S1. (PDF, 165 KB) -
No differences in the stability of wt c, Tyr577Phe mutant and p85 either in the presence or absence of GM- CSF CTL-EN cells expressing wtc or Tyr577Phe mutant were factor-deprived overnight in RPMI containing 10% FCS with no cytokine. Cells were then preincubated in 1 µg/mL cyclohexamide prior to stimulation with 1nM GM-CSF for up to 12 hours. Cell lysates were prepared at the indicated time points and subjected to SDS-PAGE and immunoblot analysis using 1 µg/mL 1C1 anti-c mAb or 1:1000 anti-p85 as described in the manuscript.
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