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Blood, Vol. 110, Issue 7, 2556-2560, October 1, 2007
The immunogenicity of Bcr-Abl–expressing dendritic cells is dependent on the Bcr-Abl kinase activity and dominated by Bcr-Abl–regulated antigens
Blood Scheich et al.
110: 2556
Supplemental materials for Scheich et al, Vol. 110, Issue 7, 2556-2560
Files in this Data Supplement:
- Document 1. Supplemental methods and materials (PDF, 87.9 KB)
- Table S1. HLA class I types of healthy donors and CML patients (PDF, 11 KB)
- Figure S1. Regulation of leukemia-associated antigens in Bcr-Abl–expressing DCs (PDF, 56.2 KB) -
(A) Kinase activity of the Bcr-Abl WT. Kinase activity of the Bcr-Abl WT construct and lack of kinase activity by the Bcr-Abl KD construct was documented by transducing LCLs (B-lymphoblastoid cell lines) with the appropriate retroviral construct. Mock transfected DCs were used as negative control. Protein expression was performed for phospho-Bcr-Abl, Bcr-Abl, and Abl by Western blot analysis. (B) DCs were transfected with mRNA coding for Bcr-Abl WT respective -KD and the expression of the leukemia-associated antigens PRAME, PR3, HAGE, and WT1 was documented at different time points after transfection.
- Figure S2. Influence of IM on maturation and priming capacities of DCs (PDF, 13.5 KB) -
Phenotype of IM pretreated mature DCs as determined by FACS analysis with mAb against CD86 (△), CD83 (○), HLA-DR (□), CD71 (■), and CD14 (●).
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