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Blood, Vol. 110, Issue 9, 3301-3309, November 1, 2007
Target proteins of C/EBP p30 in AML: C/EBPp30 enhances sumoylation of C/EBPp42 via up-regulation of Ubc9
Blood Geletu et al.
110: 3301
Supplemental materials for: Geletu et al
Files in this Data Supplement:
- Table S1. Mass spectrometry results of the target proteins of C/EBPαp30: MALDI-TOF MS (Bruker Daltonics) and MSMS analysis by AB4700 (PDF, 39.7 KB) -
(A and B) List of proteins, up and down regulated, identified in K562 C/EBP p30ER after beta estradiol induction and expression level are quantified by ProteomWeaver. (C) List of proteins regulated more than two fold and identified as common targets among the cell line and AML patient samples. (D) List of protein identified from AML patient samples with C/EBPp30 mutations.
- Figure S1. C/EBPαp30 regulates the expression of 60 different proteins (2D gel electrophoresis) (PDF, 250 KB) -
(A, B, and C) Colloidal Coomassie Blue-stained 2D gels (pH 3-10, pH 4-7, and pH 6-11) from whole cell lysates of K562 C/EBPp30-ER cells under uninduced (- -estradiol 0 h) and induced (+ -estradiol, 6 h) conditions. The encircled spots represent proteins changed in expression by C/EBPp30 and their identities are shown in Table S1A and S1B. (D & E) Colloidal Coomassie Blue-stained 2D gels pH 3-10 from two AML patient samples with C/EBPp30 mutation.
- Figure S2. Isolation of CD34+ cells from cord blood and Transfection efficiency using AMAXA (PDF, 122 KB) -
(A) CD34+ cells were isolated using Magnetic Bead separation process (Milteny) and analyzed for the surface expression of CD34 by flow cytometry. The expression of CD34+ as percentage and shown on the dot plot. (B) CD34+ cells and (C) U937 cells were transfected with eGFP plasmid using AMAXA technology for analyzing the transfection efficiency and shown eGFP-transfected CD34+ and U937 cells under normal and florescence light microscopy.
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