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Blood, Vol. 110, Issue 7, 2432-2439, October 1, 2007
Activation of plasminogen into plasmin at the surface of endothelial microparticles: a mechanism that modulates angiogenic properties of endothelial progenitor cells in vitro
Blood Lacroix et al.
110: 2432
Supplemental material for: Lacroix et al, Vol 110, Issue 7, 2432-2439
Angiogenic effect of EMP as compared to VEGF. In order to best appreciate the proangiogenic effect of low doses of MP, we compared this effect to known concentrations of vascular endothelial growth factor (VEGF) on tube formation in Matrigel. As illustrated in the Figure S1A, VEGF dose dependently enhanced tube formation to reach a maximum (50%) at 10 ng/ml. Since a maximum of 20% increase in tube formation was obtained with low doses of endothelial microparticles (EMPs) whereas increasing concentrations were inhibitory (Figure 4A), their proangiogenic effect seems to be less potent as compared to VEGF. It may be equivalent to that of 1 to 5 ng/ml VEGF. Furthermore, together with an increase in number of polygons, VEGF also induced qualitative modifications in the structure of tubes (Figure S1B).
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