|
|
Blood, Vol. 110, Issue 7, 2231-2234, October 1, 2007
Donor-derived DNA in fingernails among recipients of allogeneic hematopoietic stem-cell transplants
Blood Imanishi et al.
110: 2231
Supplemental materials for: Imanishi et al, Vol 110, Issue 7, 2231-2234
In the peripheral blood of adult T-cell leukemia/lymphoma (ATLL) patients, HTLV-1 positive T cells exist depending on the status of the disease. We collected fingernail clippers from 10 patients with ATLL along with their peripheral blood (PB). We extracted DNA from nails and white blood cells (WBCs) of PB samples, then performed genomic polymerase chain reaction (PCR) for the pX region of HTLV-1 and the  -globin gene as control following the methods we described recently (Yamasaki R, et al. Small number of HTLV-1-positive cells frequently remains during complete remission after allogeneic hematopoietic stem cell transplantation that are heterogeneous in origin among cases with adult T-cell leukemia/lymphoma. Leukemia. 2007;21:1212-1217).
Files in this Data Supplement:
- Figure S1. HTLV-1 positive cells exist in PB of ATLL patients (JPG, 37.8 KB)
-
The -globin gene was amplified in all samples using DNA extracted from PB. The pX region of HTLV-1 was detected in 9 out of 10 cases; the exception was case 8, in complete remission after allogeneic stem-cell transplantation for ATLL, reflecting the clinical situation of this case.
- Figure S2. No HTLV-1 provirus in nail of ATLL patients (JPG, 44.6 KB)
-
The -globin gene was amplified in 9 cases showing the successful extraction of DNA from 9 nail samples. However, in contrast to the PB samples, the pX region of HTLV-1 was not amplified in any case by PCR with the same condition for PB samples. M indicates molecular marker; PC, positive control; NC, negative control. Numbers under each figure represent patient case numbers.
|
|
