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Blood, Vol. 110, Issue 5, 1420-1428, September 1, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Activation of mitogen-activated protein kinase kinase (MEK)/extracellular signal–regulated kinase (ERK) signaling pathway is involved in myeloid lineage commitment Blood Hsu et al. 110: 1420 Supplemental materials for Hsu et al, Vol 110, Issue 5, 1420-1428 Files in this Data Supplement: Document 1. Supplemental methods (PDF, 88 KB) Figure S1. Sorting gates of the cells used in this study (JPG, 174 KB) - Gating procedures to FACS sort (A) CLPs, (B) CMPs, (C) VCAM-1−Flt3+ MPPs, and (D) HSCs are shown. Figure S2. Knock-down efficiency of siRNA-targeting MEK1 (JPG, 55.9 KB) - (A) GFP+ and GFP− cells were purified from splenocytes in mice reconstituted with HSCs that had been infected with siRNA-MEK1 virus (right). We also purified GFP+ cells from the mice with HSCs with empty virus (left). (B) ERK1 mRNA expression level was examined by real-time PCR. ERK1 expression in GFP− cells in gate 1 in panel A was set to 100% (column 1). Columns 2 and 3 show relative ERK1 expression in the samples from gates 2 and 3, respectively, in panel A. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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