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Blood, Vol. 110, Issue 9, 3245-3252, November 1, 2007 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Key differences in TLR3/poly I:C signaling and cytokine induction by human primary cells: a phenomenon absent from murine cell systems Blood Lundberg et al. 110: 3245 Supplemental materials for: Lundberg et al Files in this Data Supplement: Figure S1. Poly I:C fail to induce IL-10 in human dendritic cells (JPG, 33.9 KB) - Human DC were stimulated with increasing concentrations of either LPS or poly I:C as indicated. The supernatants were collected and analyzed for IP-10 by ELISA. The data are mean values (±) SD. Figure S2. Poly I:C-induced ERK activation in RA synovial fibroblasts is not mediated by a secondary soluble mediator (JPG, 27.1 KB) - Fibroblasts were isolated from synovial tissue from RA patients undergoing joint replacement. The cells were pretreated with 10 ug/ml of Brefeldin A for 1 h followed by stimulation of 20 ug/ml poly I:C for either the indicated times and analyzed for ERK phosphorylation and -actin by western blot, or stimulated for 18 h and analyzed for the production of IL-6 by ELISA. Figure S3. Stimulation of human dendritic cells with Poly I:C induces a rapid nuclear translocation of IRF-1 (JPG, 46.5 KB) - Human DC were either left untreated (Un) or stimulated for the indicated times with poly I:C or LPS and were subsequently lysed to obtain nuclear extracts. The samples were examined for the level of IRF-1 and topoisomerase by Western blotting. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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