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Blood, Vol. 110, Issue 13, 4480-4491, December 15, 2007
Regulation of Fc R-stimulated phagocytosis by the 72-kDa inositol polyphosphate 5-phosphatase: SHIP1, but not the 72-kDa 5-phosphatase, regulates complement receptor 3–mediated phagocytosis by differential recruitment of these 5-phosphatases to the phagocytic cup
Blood Horan et al.
110: 4480
Supplemental materials for: Horan et al
Files in this Data Supplement:
- Video 1. Supplement to Figure 3D (MOV, 236 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with GFP-72-D480N5ptase and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 10 frames/sec. Green represents GFP-72-D480N5ptase localization during phagocytosis.
- Video 2. Supplement to Figure 4A (MOV, 522 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with vector and GFP-ARNO/PH and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 10 frames/sec. Green represents GFP-ARNO/PH localization during phagocytosis.
- Video 3. Supplement to Figure 4B (MOV, 334 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with wild-type HA-72-5ptase and GFP-ARNO/PH and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 10 frames/sec. Green represents GFP-ARNO/PH localization during phagocytosis.
- Video 4. Supplement to Figure 4C (MOV, 127 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with HA-72-D480N5ptase and GFP-ARNO/PH and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 5 frames/sec. Green represents GFP-ARNO/PH localization during phagocytosis.
- Video 5. Supplement to Figure 5A (MOV, 133 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with vector and YFP-TAPP1/PH and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 10 frames/sec. Green represents YFP-TAPP1/PH localization during phagocytosis.
- Video 6. Supplement to Figure 5B (MOV, 93.5 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with wild-type HA-72-5ptase and YFP-TAPP1/PH and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 10 frames/sec. Green represents YFP-TAPP1/PH localization during phagocytosis.
- Video 7. Supplement to Figure 5C (MOV, 136 KB)
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Phagocytosis was stimulated by the addition of eIgG to RAW264.7 macrophages transfected with HA-72-D480N5ptase and YFP-TAPP1/PH and phagocytosis monitored using the FV1000 confocal microscope. Images were collected every 12.5 sec at 37°C and displayed at 10 frames/sec. Green represents YFP-TAPP1/PH localization during phagocytosis.
- Video 8. Supplement to Figure 6B (MOV, 214 KB)
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Phagocytosis was stimulated by the addition of serum-opsonized zymosan to mouse macrophages isolated from SHIP1+/+AktPH-GFP Tg and phagocytosis monitored using a DM IRE2 microscope (Leica) fitted with a confocal imaging system (Yokogawa). Images were collected every 10 sec at 37°C and displayed at 6 frames/sec. Green represents AktPH-GFP localization during phagocytosis.
- Video 9. Supplement to Figure 6B (MOV, 830 KB)
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Phagocytosis was stimulated by the addition of serum-opsonized zymosan to mouse macrophages isolated from SHIP−/−AktPH-GFP Tg and phagocytosis monitored using a DM IRE2 microscope (Leica) fitted with a confocal imaging system (Yokogawa). Images were collected every 10 sec at 37°C and displayed at 6 frames/sec. Green represents AktPH-GFP localization during phagocytosis in the absence of SHIP1.
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