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Blood, Vol. 110, Issue 8, 3049-3055, October 15, 2007
Normal erythropoiesis but severe polyposis and bleeding anemia in Smad4-deficient mice
Blood Pan et al.
110: 3049
Supplemental materials for Pan et al, Vol. 110, Issue 8, 3049-3055.
Files in this Data Supplement:
- Figure S1. Genomic structure of Smad4 conditional knockout allele (JPG, 64.7 KB).
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The probe used for Southern blot is indicated by a thick horizontal line. (A) The wild type allele of Smad4 with EcoRV sites located in introns 7 and 9 is shown. (B) The conditional allele (fl) with insertion of two loxP sites (triangles) and an additional EcoRV site. The position of primers used for genotyping is shown (S1-S4). (C) The deleted allele after Cre recombination ( ). S5-S6, position of primers used for detecting the deleted allele. (D) Southern blot of DNA from bone marrow digested with EcoRV is shown. Arrows indicate the positions of the wild type allele (wt), the floxed conditional allele (fl), and the deleted allele after Cre recombination (). (E) Efficiency of Cre/loxP mediated recombination in VavCre; Smad4fl/fl mice. Representative PCR analysis of DNA from peripheral blood cells of VavCre; Smad4fl/fl (/), VavCre; Smad4fl/+ (
/+) and Smad4fl/+ (fl/+) mice. Primers S5S6 detect the deleted allele, while primers S1-S2 detect the floxed and the wild type alleles. Note the absence of floxed allele in S1-S2 PCR with DNA from / mice.
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