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Blood, Vol. 110, Issue 3, 1055-1063, August 1, 2007
Alternative splicing due to an intronic SNP in HMSD generates a novel minor histocompatibility antigen
Blood Kawase et al.
110: 1055
Supplemental materials for Kawase et al, Vol 110, Issue 3, 1055-1063
Files in this Data Supplement:
- Figure S1. Specific lysis by 2A12-CTL of the CD 34+ CD38− fraction of primary AML cells (JPG, 45.1 KB)
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The cytolytic activity of CTL-2A12 was evaluated in a 51Cr release assay. The CD34+ CD38− fraction of three primary acute myeloid leukemia (AML) samples (AML-2, -3 and -4) shown in Figure 6A were sorted using a flow cytometer. The sorted CD34+ CD38− fraction represented 2% to 4 % of the CD34+ cells. Cells were labeled with 51Cr for 16 hours. Target cells were cocultured with 2A12-CTL at the indicated E/T ratios and the supernatants were harvested and counted at 4 hours.
- Figure S2. Specific lysis by 2A12-CTL of the CD138+ fraction of primary multiple myeloma cells (JPG, 38 KB)
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The cytolytic activity of CTL-2A12 was evaluated in a standard 4 hour 51Cr release assay. The CD138+ fraction of two primary myeloma cells, MM-1 (HLA-B*4403+ and ACC6+) and MM-2 (HLA-B*4403+ but ACC6−) were sorted using immunomagnetic bead-based selection. Cells were labeled with 51Cr for 4 hours. Target cells were cocultured with 2A12-CTL at the indicated E/T ratios and the supernatants were harvested and counted.
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