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Blood, Vol. 111, Issue 3, 1504-1511, February 1, 2008
Dendritic cells loaded with apoptotic antibody-coated tumor cells provide protective immunity against B-cell lymphoma in vivo
Blood Franki et al.
111: 1504
Supplemental materials for: Franki et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 90.1 KB)
- Figure S1. Antibody staining of irradiated 38C13 lymphoma cells (PDF, 77.4 KB) -
Irradiated (30 Gy) and non-irradiated 38C13 tumor cells were incubated 30 minutes on ice with either media alone, control mouse IgG2a mAb, or anti-idiotype antibody. After washing away unbound antibody cells were analyzed via flow cytometry for (A) bound IgG or (B) surface IgM. Filled in line represents unstained cells, thin grey line represents isotype control staining, and thick black line represents (A) anti-IgG FITC staining or (B) anti-IgM PE staining. (B) Irradiated cells were also cultured overnight at 37°C after S1C5 mAb incubation and surface IgM detected via flow cytometry.
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