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Blood, Vol. 110, Issue 5, 1639-1647, September 1, 2007
MN1 overexpression induces acute myeloid leukemia in mice and predicts ATRA resistance in patients with AML
Blood Heuser et al.
110: 1639
Supplemental materials for Heuser et al, Vol. 110, Issue 5, 1639-1647
Files in this Data Supplement:
- Figure S1. Retroviral vectors used to express MN1 and GFP in murine BM (PDF, 12.5 KB) -
LTR indicates long terminal repeat; R, U5, elements within LTR; IRES, internal ribosomal entry site; EGFP, enhanced green fluorescent protein, PRE, post-transcriptional regulatory element. VP16 was cloned in frame to the C-terminus of MN1.
- Figure S2. GFP expression in nontransduced and transduced BM cells (PDF, 360 KB) -
(A) Nontransduced cells. (B) pSF91GFP-transduced cells 4 days after the end of infection. (C) pSF91MN1-transduced cells 4 days after the end of infection. (D) pSF91MN1-transduced cells 30 days after infection.
- Figure S3. In vitro growth analysis of unsorted bone marrow cells transduced with MN1-GFP, and monitored for GFP expression over 17 days (PDF, 11.7 KB) -
Means plus or minus the SD of 4 independent experiments are shown.
- Figure S4. Immunophenotyping of leukemic BM cells (PDF, 108 KB) -
Expression of the indicated cell-surface antigen is shown on the x-axis and the expression of GFP is shown on the y-axis.
- Figure S5. Transplantability of MN1 leukemias to secondary recipients (PDF, 18.3 KB) -
Survival curve for mice transplanted with MN1-transduced cells (primary transplants, n = 18; secondary recipients, n = 5).
- Figure S6. Proliferation inhibition of ND13CTL and ND13MN1 cells upon treatment with different ATRA concentrations (PDF, 17.5 KB) -
Means plus or minus the SD of 3 independent experiments are shown. # P=.026 at 24 hours, *P<.001 at 48, 72, 96hours.
- Figure S7. Immunophenotype of ND13CTL vs. ND13MN1 cells (PDF, 11.9 KB) -
Means plus or minus the SD of 3 independent experiments are shown; all P<.001.
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